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KMID : 0613820020120030264
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Journal of Life Science
2002 Volume.12 No. 3 p.264 ~ p.273
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Cloning and Expression of Antifungal Protein (PR5) Genes from Hot Pepper (Capsicum annuum L.)
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Park Hae-Jin
Lee Jung-Hoon
Yoon Yong-Hwi
Kim Hak-Yoon
Shin Dong-Hyun
Lee Jin-Hyung
Kim Dal-Ung
Kim Kil-Ung
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Abstract
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We have isolated and artificially expressed three cDNA clones of Capsicum annuum PR5 genes for elucidating the antifungal activity against Phytophthora capsici which contracted a hot pepper root rot in field condition. Three divergent PR5 proteins from hot pepper were designated as CAPR5-1 and CAPR5-2 from susceptible cultivar (Subicho) as well as CAPR5-3 from resistant cultivar (CM331) in response to P. capsici. The cDNA similarity was found over 80% of identity among the three CAPR5s, and deduced amino acid sequence was characterized that all of CAPR5s contained 16 cysteine residues which possibly had a significant role in the structural formation. The result of genomic DNA blot showed that CAPR5-1 and CAPR5-2 existed as single copy in the Subicho genome. Three recombinant CPARs in E. coli were identified by SDS-PAGE, and each expressed protein was treated on the PDA medium which contained cultured pathogens. Although three CAPR5 proteins did not affected the hyphal growth of Glomerella glycines and Colletotrichum lagenarium, CAPR5-1, CAPR5-2, and CAPR5-3 showed a specific antifungal activities against P. capsici.
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KEYWORD
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Hot pepper, Osmotin-like protein, Pathogenesis-related protein
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